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Multilocus <t> PCR-RFLP </t> genotyping of <t> T. gondii </t> from animals and humans in China.
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Image Search Results


Multilocus  PCR-RFLP  genotyping of  T. gondii  from animals and humans in China.

Journal: PLoS ONE

Article Title: Genotypes and Mouse Virulence of Toxoplasma gondii Isolates from Animals and Humans in China

doi: 10.1371/journal.pone.0053483

Figure Lengend Snippet: Multilocus PCR-RFLP genotyping of T. gondii from animals and humans in China.

Article Snippet: The parasite burdens in blood and tissues during acute and chronic phase of infection were assessed in 96-wells plates using an ABI 7300 Sequence Detection System (Applied Biosystems, USA) and T. gondii PCR fluorescence diagnostic kit (DAAN Gene, China).

Techniques:

Kinetics of infection in blood and organs after oral infection with 50 cysts of TgCtwh6 isolate (genotype Chinese 1) in mice. I: the number of DNA copies in blood and tissues of the TgCtwh6 isolate post infection at various intervals by qPCR. Each point represents the mean value of the T. gondii DNA copies for five mice ± SD. II: detection of PCR products of T. gondii 529 bp fragments extracted from brain or ascitic fluid in the recipient mice. Abbreviations: A ; blood, B ; heart, C ; liver, D ; brain, and E ; lymph node. b, n, and p represent blank, negative and positive control. * P <0.05.

Journal: PLoS ONE

Article Title: Genotypes and Mouse Virulence of Toxoplasma gondii Isolates from Animals and Humans in China

doi: 10.1371/journal.pone.0053483

Figure Lengend Snippet: Kinetics of infection in blood and organs after oral infection with 50 cysts of TgCtwh6 isolate (genotype Chinese 1) in mice. I: the number of DNA copies in blood and tissues of the TgCtwh6 isolate post infection at various intervals by qPCR. Each point represents the mean value of the T. gondii DNA copies for five mice ± SD. II: detection of PCR products of T. gondii 529 bp fragments extracted from brain or ascitic fluid in the recipient mice. Abbreviations: A ; blood, B ; heart, C ; liver, D ; brain, and E ; lymph node. b, n, and p represent blank, negative and positive control. * P <0.05.

Article Snippet: The parasite burdens in blood and tissues during acute and chronic phase of infection were assessed in 96-wells plates using an ABI 7300 Sequence Detection System (Applied Biosystems, USA) and T. gondii PCR fluorescence diagnostic kit (DAAN Gene, China).

Techniques: Infection, Positive Control

Panel A: a map of China to show the cumulative data of geographic distribution where the Chinese isolates of T. gondii used for genotyping were collected. The provinces and cities where T. gondii isolates were obtained and indicated by red dots and black dots, respectively. Red dots represent the locations (counties) where the isolates were collected in this work. Black dots indicate the locations (counties) where the isolates were collected in the early studies. SX: Shanxi province; SD: Shandong province; HN: Henan province; AH: Anhui province; JS: Jiangsu province; HuB: Hubei province; HuN: Hunan province; GD: Guangdong province; ZJ: Zhejiang province; FJ: Fujian province; YN: Yunnan province; QH: Qinghai province; GS: Gansu province; XJ: Xinjiang province; BJ: Beijing city; SH: Shanghai city. Panel B: a: the cumulative data of genotypes of T. gondii Chinese isolates. b: the genotypes of T. gondii isolates from human patients in mainland China. This is the first report of the genotype ToxoDB#204 from humans.

Journal: PLoS ONE

Article Title: Genotypes and Mouse Virulence of Toxoplasma gondii Isolates from Animals and Humans in China

doi: 10.1371/journal.pone.0053483

Figure Lengend Snippet: Panel A: a map of China to show the cumulative data of geographic distribution where the Chinese isolates of T. gondii used for genotyping were collected. The provinces and cities where T. gondii isolates were obtained and indicated by red dots and black dots, respectively. Red dots represent the locations (counties) where the isolates were collected in this work. Black dots indicate the locations (counties) where the isolates were collected in the early studies. SX: Shanxi province; SD: Shandong province; HN: Henan province; AH: Anhui province; JS: Jiangsu province; HuB: Hubei province; HuN: Hunan province; GD: Guangdong province; ZJ: Zhejiang province; FJ: Fujian province; YN: Yunnan province; QH: Qinghai province; GS: Gansu province; XJ: Xinjiang province; BJ: Beijing city; SH: Shanghai city. Panel B: a: the cumulative data of genotypes of T. gondii Chinese isolates. b: the genotypes of T. gondii isolates from human patients in mainland China. This is the first report of the genotype ToxoDB#204 from humans.

Article Snippet: The parasite burdens in blood and tissues during acute and chronic phase of infection were assessed in 96-wells plates using an ABI 7300 Sequence Detection System (Applied Biosystems, USA) and T. gondii PCR fluorescence diagnostic kit (DAAN Gene, China).

Techniques: